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. 2006 Feb;74(2):910–919. doi: 10.1128/IAI.74.2.910-919.2006

FIG. 6.

FIG. 6.

3-oxo-C12-HSL inhibits agr expression. (A) Northern blot analysis of RNAIII transcripts prepared from S. aureus RN6390 grown for 4, 6, or 9 h in the absence of 3-oxo-C12-HSL (lanes 0) or in the presence of 3-oxo-C12-HSL at a concentration of 2.5 μM (lanes 2.5) or 5 μM (lanes 5). The top panel shows the loading gel with the positions of the 23S and 16 S rRNAs indicated, and the bottom panel shows the Northern blot. (B) Northern blot analysis of RNAIII transcripts prepared from S. aureus RN6390 grown for 3.5 h to induce agr prior to addition of no 3-oxo-C12-HSL (lanes 0) or 3-oxo-C12-HSL at a concentration of 5 μM (lanes 5) and sampled every hour for a further 5 h. The top panel shows the loading gel with the positions of the 23S and 16S rRNAs indicated, and the bottom panel shows the Northern blot. (C) Dose-response curve showing the inhibition of an S. aureus agrP3::blaZ reporter by 3-oxo-C12-HSL. The error bars indicate standard deviations (n = 3).