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Induction of the 17b epitope by sCD4. Binding of the 17b antibody to gp160ΔCT glycoprotein on 293T cells and that on PLs were compared. Cells and PLs were incubated with (+) and without (−) sCD4 prior to binding of the 17b antibody and analyzed by FACS. Staining without preincubation with sCD4 was set as 100%, and increase of 17b binding is shown for 293T cells (open bars) and PLs (solid bars). MFI, mean fluorescence intensity.
