TABLE 2.
Plasmids used in this study
| Plasmid | Relevant characteristic(s)a | Source or reference |
|---|---|---|
| pGEM-3Zf(−) | Ampr | Promega |
| pTO2 | pCRT7/CT-TOPO containing the hmuR gene with the signal peptide sequence, Ampr | 49 |
| pTO2-I | pCRT7/CT-TOPO containing the hmuRH95A gene with the signal peptide sequence, Ampr | This study |
| pTO2-II | pCRT7/CT-TOPO containing the hmuRH434A gene with the signal peptide sequence, Ampr | This study |
| pTO2-I+II | pCRT7/CT-TOPO containing hmuRH95A,H434A gene with the signal peptide sequence, Ampr | This study |
| pTO2-III | pCRT7/CT-TOPO containing the hmuRYRAP420-423YAAA gene with the signal peptide sequence, Ampr | This study |
| pTO2-IV | pCRT7/CT-TOPO containing the hmuRNPDL442-445NAAA gene with the signal peptide sequence, Ampr | This study |
| pWS1 | pGEM-3Zf(−) containing 485-bp N-terminal region of the hmuR gene and ermF cassette within the PstI site of the hmuR gene | 49 |
| pI | pGEM-3Zf(−) containing hmuRH95A, ermF, and DSR | This study |
| pII | pGEM-3Zf(−) containing the C-terminal region of the hmuRH434A gene, ermF, and DSR | This study |
| pI + II | pGEM-3Zf(−) containing hmuRH95A,H434A, ermF, and DSR | This study |
| pIII | pGEM-3Zf(−) containing the C-terminal region of the hmuRYRAP420-423YAAA gene, ermF, and DSR | This study |
| pIV | pGEM-3Zf(−) containing the C-terminal region of the hmuRNPDL442-445NAAA gene, ermF, and DSR | This study |
a
ermF cassette, Bacteroides fragilis ermF encoding erythromycin resistance gene; DSR, downstream region (254 bp) of the hmuR gene.