FIG. 1.

Sites of amino acid insertions and deletions in espB mutants in relation to FAS activity and to sequence features of EspB. The amino acid sequence of EspB from EPEC strain E2348/69 is shown. Residues conserved among all published EspB sequences are shown in red. Domains predicted to form coiled-coil interactions are shaded in gray. A predicted transmembrane domain is shaded in yellow. The last amino acids before the sites of linker insertion mutations that interfere (red) or do not interfere (green) with overall EspB function are indicated with inverted triangles. Amino acids deleted in certain espB mutants are underlined and color-coded in the same manner as insertion mutations.