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Immunoblot of EspB from culture supernatant (A) and bacterial pellets (B) of selected strains. Lane 1, espB mutant UMD864; lane 2, wild-type EPEC strain E2348/69; lanes 3 to 13, UMD864 complemented with plasmids as indicated. The positions of molecular mass markers (in kilodaltons) are shown. In panel B, a nonspecific cross-reactive band migrating slower than EspB serves as a loading control.
