Hemolytic activity of espB mutants. The percent lysis of sheep erythrocytes after incubation with each mutant and controls is indicated. Strains tested include the wild-type strain E2348/69, the espB mutant UMD864, the espD mutant UMD870, the espA mutant UMD872, and the espB mutant containing plasmids as shown. Green columns represent FAS-negative strains, red columns represent FAS-positive strains, and the gray column represents a mutant with an intermediate FAS phenotype. Data are from five independent experiments. Error bars represent the standard errors of the means.