TABLE 2.

Plasmids containing mutated espB alleles and A/E and expression-secretion phenotypes conferred by the mutated alleles generated in this study^a

Plasmid	Type of mutation	FAS resultb	Secretion phenotypec
pEspB4	5-Codon insertion	+	+
pEspB5/30	Deletion	−	−
pEspB11	5-Codon insertion	+	+
pEspB13	5-Codon insertion	+	+
pEspB25	5-Codon insertion	+	+
pEspB30	5-Codon insertion	+	+
pEspB36	5-Codon insertion	+	+
pEspB37	31-Codon insertion	+	+
pEspB48	5-Codon insertion	−	+
pEspB60	31-Codon insertion	−	+
pEspB68	5-Codon insertion	−	+
pEspB70	5-Codon insertion	−	+
pEspB75	31-Codon insertion	−	+
pEspB78	31-Codon insertion	−	+
pEspB83	5-Codon insertion	−	−
pEspB98	5-Codon insertion	−	+
pEspB101	5-Codon insertion	−	+
pEspB128	5-Codon insertion	+	+
pEspB166	31-Codon insertion	−	+
pEspB173	5-Codon insertion	−	−
pEspB179	5-Codon insertion	+	+
pEspB180/203	Deletion	+	+
pEspB184	5-Codon insertion	+	+
pEspB186	5-Codon insertion	+	+
pEspB188	5-Codon insertion	+	+
pEspB203	5-Codon insertion	+	+
pEspB239	5-Codon insertion	−	+
pEspB241	5-Codon insertion	+/−	+
pEspB247	5-Codon insertion	+	+
pEspB252	5-Codon insertion	+	+
pEspB254	5-Codon insertion	−	+
pEspB257	5-Codon insertion	−	+
pEspB260	5-Codon insertion	−	+
pEspB264	31-Codon insertion	−	+
pEspB268	31-Codon insertion	−	−
pEspB282	31-Codon insertion	−	+
pEspB289	5-Codon insertion	−	+
pEspB302	5-Codon insertion	−	−
pEspB304stop	Deletion	−	+
pEspB305	31-Codon insertion	−	+
pEspB316	5-Codon insertion	+	+
pEspB316stop	Deletion	+	+

^aThe precise positions of the mutations are indicated by the plasmid names as described in the text.

^b+, able to restore a positive FAS result to an espB null mutant; −, unable to do so; +/−, able to confer very weak and inconsistent actin staining.

^c+, detectable levels of protein are expressed and secreted; −, detectable levels of protein are neither expressed nor secreted.