TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Description | Reference or source |
|---|---|---|
| S. pneumoniae strains | ||
| TIGR4 | Serotype 4 meningeal isolate | 28 |
| TIGR4X | Bioluminescent TIGR4 bearing Tn4001 luxABCDE Kmr | 21 |
| CbpG-X | Bioluminescent CbpG-derivative of TIGR4X | This study |
| CbpG-KKGX | Bioluminescent TIGR4X derivative expressing CbpG amino acids 1 to 215 | This study |
| TIGR4R | Unencapsulated derivative of TIGR4 | 12 |
| CbpG-KKGR | Derivative of TIGR4R expressing CbpG amino acids 1 to 215 | 11 |
| CbpG-R | Derivative of TIGR4R deficient in entire CbpG expression | This study |
| CbpGHK-R | Derivative of TIGR4R deficient in SP0386 histidine kinase | This study |
| CbpF-R | Derivative of TIGR4R deficient in CbpF expression | 11 |
| E. coli strains | ||
| XL-1 Blue | Host strain for construction of recombinant plasmids | Stratagene |
| Top10F′ | Host strain for TA cloning of recombinant plasmids | Invitrogen |
| BL21 (DE3) | Expression host for recombinant protein purification | Invitrogen |
| L. casei ATCC 393 | Host strain for Lactobacillus expression | 19 |
| Plasmids | ||
| pCR2.1 | TA cloning vector | Invitrogen |
| PET32A | Expression vector with a thioredoxin fusion tag | Novagen |
| pET43G24-184 | pET43a vector expressing CbpG amino acids 24 to 184 | This study |
| pCAGGS | Eukaryotic expression vector | A. Portner |
| pLPMSSA3 | Lactobacillus expression vector | 1 |
| pLPMSSA4 | Lactobacillus expression vector with BamHI cloning site | This study |
| pLPMSSA4 (CbpG) | pLPMSSA4 vector expressing CbpG amino acids 1 to 285 | This study |