FIG. 5.
β1 integrin localization with EHEC O157:H7, detected by immunofluorescence of infected tissue sections. Sections of EHEC O157:H7 Strr-infected intestine corresponding to those depicted in Fig. 3 and 4 were immunostained with anti-β1 integrin and anti-O157 antisera. Images for mouse (top row), calf (second row), and pig (third row) are shown for stained O157 (first column) and stained β1 integrin (second column). Images in the bottom row were taken from a piglet infected with the normal enteric E. coli strain 123. The fluorescent images were overlaid (third column) to demonstrate accumulated β1 integrin at the site of EHEC O157:H7 adherence. Phase-contrast micrographs overlaid with the combined fluorescent images (fourth column) depict the orientation of the epithelial cell (E) and lumen (L). Representative adherent bacteria and associated immunostained β1 integrin are indicated by blue arrows. Bar, 1 μm.