FIG. 6.

Quantitative analysis of the fluorescence signal intensity obtained from digitized images of stained tissue sections. The sum of the fluorescence signal was recorded for 1 square μm at positions with (+) and without (−) adherent EHEC O157:H7 in images such as those presented in Fig. 3, 4, and 5. The solid bars represent the means of 100 observations taken from different microscopic fields of sections from pig, calf, or mouse stained for Tir (A), β1 integrin (B), or nucleolin (C). Values were normalized to the positions without adherent bacteria. Error bars represent the normalized 95% confidence intervals of the means.