FIG. 1.
SOS-induced increase in ctxA transcript abundance is dependent on PrstA activity. pIIICTX and ctxA transcripts levels were measured by quantitative RT-PCR. Fold increases were calculated by dividing the amount of mRNA measured in MMC-treated cells by the amount of mRNA measured in untreated cells. In the schematic diagram of the CTX prophage, the lollipop symbol above rstA indicates the site of the terminator in BD899. N16961 (WT) and BD899 were treated with 20 ng/ml of mitomycin C for 3 h. The data are data from one representative experiment.