FIG. 3.

Expression of interleukin-18 by monocyte-derived macrophages from Salmonella-resistant and -susceptible inbred chicken lines challenged with Salmonella serovar Gallinarum (A) or Salmonella serovar Typhimurium (B) at an MOI of 10. Expression was determined by qRT-PCR from RNA isolated in triplicate challenges from three repeats of macrophages pooled from four different birds for each repeat. Significant differences in expression between chicken lines at a particular time point (P ≤ 0.05) are indicated by an asterisk; highly significant differences (P ≤ 0.01) are indicated by a double asterisk (n = 9). Error bars indicate standard errors of the means.