TABLE 1.
Bacterial strains and plasmids
| Name | Relevant genotype, phenotype, or description | Source/referencea |
|---|---|---|
| Strains | ||
| DH5αλpir | pir Nalr | IAH |
| SY327αλpir | pir Nalr | IAH/22 |
| SM10λpir | pir traRP4 | IAH/36 |
| WS-4437A | O128:H12 CFA/I ST EAST1 LT (wild-type ETEC) | NMRC/this work |
| WS-1858B | O71:H− CFA/I ST EAST1 Apr Tpr Sur (wild-type ETEC) | NMRC/this work |
| A18-34 | O71:H− CFA/I ΔestA EAST1 Apr Tpr Sur | This work |
| A18-34Aps | O71:H− CFA/I ΔestA EAST1 Tpr Sur | This work |
| A18-34ApsTps | O71:H− CFA/I ΔestA EAST1 | This work |
| A18-34ApsTps ΔastA | O71:H− CFA/I ΔestA ΔastA | This work |
| ACAM2010(pSTREP) | O71:H− CFA/I ΔestA ΔastA ΔaroC ΔompC ΔompF Smr | This work |
| E1392/75 | O6:H16 CS1 CS3 ST LT Sur Smr | 40 |
| E1392/75-2A | O6:H16 CS1 CS3 Sur Smr | 40 |
| PTL003 | O6:H16 CS1 CS3 Sur Smr ΔaroC ΔompC ΔompF | 42 |
| Plasmids | ||
| RP4 | 60-kb plasmid used as cccDNA size standard | IC/23 |
| pvirF98 | ∼90-kb virulence plasmid from serovar Typhimurium strain F98 used as cccDNA size standard | IC |
| ColVIk94 | ∼127-kb plasmid used as cccDNA size standard | IC |
| pACYC-Tc | ori15A Tcr | This work |
| pDM4 | oriR6K mobRP4 sacB cat | IAH/31 |
| pDM4A7 | oriR6K mobRP4 cat | This work |
| pDM4A7ΔEco | oriR6K mobRP4 cat | This work |
| pJCB10 | oriR6K mobRP4 cat | This work |
| pJCB12 | oriR6K mobRP4 sacB cat | This work |
| pJCB12-estA | 345-bp fragment, including the whole estA gene, in pJCB12 | This work |
| pJCB12-ΔastA | 286-bp fragment, incorporating a 62-bp defined deletion in astA gene, in pJCB12 | This work |
| pJCB12-ΔaroC345 | 748-bp fragment, incorporating a 345-bp defined deletion in aroC, in pJCB12 | This work |
| pJCB12-ΔompC | 1,016-bp fragment, incorporating a defined deletion of the whole ompC gene, in pJCB12 | This work |
| pJCB12-ΔompF | 943-bp fragment, incorporating a defined deletion of the whole ompF gene, in pJCB12 | This work |
| pJCB12-ori15A | pACYC-Tc replication origin in pJCB12 | This work |
a
IAH, provided by P. Barrow, Institute for Animal Health, Compton, United Kingdom; NMRC, provided by S. Savarino, Naval Medical Research Center; IC, provided by D. Pickard, Imperial College, London, United Kingdom.