TABLE 1.
Oligonucleotides used for lambda Red-mediated recombination
| Deletion | Template | Primer | Oligonucleotide sequence (5′ to 3′) |
|---|---|---|---|
| Δ1::kan | pKD4 (kan) | Δ1-P1 | TGATAGAGCTTATCTATATAAGGTATAAGGTGCTGAAAATGTGTAGGCTGGAGCTGCTTC |
| Δ1-P2 | TTACATGTTGAATTTGAAGGCGAGTGGACCGGACCTTATCATATGAATATCCTCCTTAGT | ||
| Δ2::kan | pKD4 (kan) | Δ2-P1 | AGGTGAAATATGACGACACGGTATTCTCGTAGTGAACGGTGTGTAGGCTGGAGCTGCTTC |
| Δ2-P2 | GAGTCTGCTCCTCATATAAATTGAGAGAATTAGGATGAACATATGAATATCCTCCTTAGT | ||
| Δ3::kan | pKD4 (kan) | Δ3-P1 | GTTTAATTCTATAAAAGAAAAACGTACGATATCCATTAATGTGTAGGCTGGAGCTGCTTC |
| Δ3-P2 | CAAAGATTTGATGGGGAACTCGCTCATCAACACGGCTGCCATATGAATATCCTCCTTAGT | ||
| Δ4::dhfr | EZ::TN<DHFR>a | Δ4-P1 | TTTTTGATAGCGATATCCATCCCGCCAGGTTGGATACGGATAGACGGCATGCACGATTTG |
| Δ4-P2 | TAACTTTTACTGAGCGAAATCTGATATTACTGGCACCACCGCATCCAATGTTTCCGCCAC | ||
| Δ2B::kan | pKD4 (kan) | Δ2B-P1 | AGGTGAAATATGACGACACGGTATTCTCGTAGTGAACGGCGCTGCCGCAAGCACTCAGGG |
| Δ2B-P2 | GAGTCTGCTCCTCATATAAATTGAGAGAATTAGGATGAAGTCCCGCTCAGAAGAACTCGT | ||
| Δ3B::cat | pKD3 (cat) | Δ3B-P1 | TGACTTCGGGCAAATTCTGCCGGAGTCAGGTTATTTAACCTTCGGAATAGGAACTTCATT |
| Δ3B-P2 | CGTTTAATCTGCGCCCTGAGCGACTGGATTTCACGTTGCGGCGCGCCTACCTGTGACGGA | ||
| Δhha | pKD4 (kan) | Δhha-P1 | TATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGTGTGTAGGCTGGAGCTGCTTC |
| Δhha-P2 | ATTCATGGTCAATTCGGCGAGGCGGTGATCTGCGGCTGACATATGAATATCCTCCTTAGT |
a
Obtained from Epicentre.