FIG. 1.

Appearance of unusual subsets of γδ T cells elicited by microbial infection in the absence of Vγ1⁺ T cells. (A) Profile of TCR-Vγ chain expression in the spleens of wild-type (C57BL/6) and TCR-Vγ1^−/− mice 8 days after infection with Listeria, as determined by RT-PCR analysis. The results shown are representative of those obtained from more than 10 mice of each strain. (B) Representative flow cytometric staining profiles of γδ T cells expressing Vγ4, Vγ5, or Vγ7 receptors among spleen cells recovered from TCR-Vγ1^−/− mice 8 days after Listeria infection (n = 10). Percentages of γδ cells positive for each marker are shown on the dot plots.