FIG. 2.

Effects of DC supplementation on vaccine-specific cytokine production in adults. PBMC from adults (n = 23) were cultured for 48 h with diphtheria toxoid (diptox) or tetanus toxoid (tettox) in the presence or absence of additional autologous DC. (A) Data are expressed as Δ values, designating levels of cytokine production above those in unstimulated control cultures. Error bars indicate 95% confidence intervals. (B) Percentages of subjects with positive cytokine responses to vaccine antigens. The data shown in panel A were analyzed in order to show the percentage of subjects manifesting at least one detectable cytokine response following in vitro stimulation with diphtheria toxoid or tetanus toxoid. *, P < 0.05; **, P < 0.005 (Wilcoxon signed rank test).