Effects of DC supplementation on CD4+ T-cell apoptosis by tetanus toxoid-stimulated PBMC from 12-month-old children (n = 7) and adults (n = 7). Using flow cytometry, apoptotic CD4+ T cells were identified as annexin V-positive, propidium iodide-positive cells and the data expressed as a percentage of total PBMC. Ag, antigen. Error bars indicate 95% confidence intervals. a, P = 0.009; b, P = 0.02; c, P = 0.012 (Wilcoxon signed rank test).