TABLE 2.
Results of the in situ hybridization technique to detect PCV2 nucleic acid
| Inoculum | Pig | Score for the following samplea:
|
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Lungs | Tonsils | Inguinal LN | Mesenteric LN | Spleen | Peyer's patches | Thymus | Liver | Kidneys | Nasal turbinates | ||
| PCV2 | 1 | − | + | + | − | + | + | − | − | − | − |
| 2 | − | − | + | − | − | − | − | − | − | − | |
| 3 | − | + | − | − | − | − | − | − | − | − | |
| 4 | − | − | ++ | + | − | − | − | − | − | − | |
| 5 | − | − | − | − | − | − | − | − | − | − | |
| 6 | − | + | + | − | − | − | − | − | − | − | |
| 7 | − | + | + | + | + | + | − | − | − | − | |
| Total | 0 | 4 | 5 | 2 | 2 | 2 | 0 | 0 | 0 | 0 | |
| PRRSV and PCV2 | 8 | − | + | + | ++ | − | − | − | − | − | − |
| 9 | ++ | +++ | +++ | +++ | + | ++ | − | + | + | + | |
| 10 | − | − | + | + | − | − | − | − | − | − | |
| 11 | − | − | + | − | + | − | − | − | − | − | |
| 12 | − | − | + | + | + | − | − | − | + | − | |
| 13 | − | + | + | + | + | − | − | − | − | − | |
| 14 | ++ | +++ | +++ | +++ | +++ | +++ | +++ | + | + | − | |
| Total | 2 | 4 | 7 | 6 | 5 | 2 | 1 | 2 | 3 | 1 | |
a
LN, Lymph nodes; −, absence of PCV2 nucleic acid; +, slight presence of PCV2 nucleic acid; ++, moderate presence of PCV2 nucleic acid; +++, strong presence of PCV2 nucleic acid.