FIG. 5.

Selection pressures for NP1-SM and NP1-MC differ. (a) Cocultivation in a macrophage cell line demonstrated enhanced intracellular survival of the NP1-MC variant after 16 and 36 h compared to that of NP1-SM. Black bars denote yeast cells grown in the presence of macrophages that are not intracellular (no antibody added). White bars reflect the percentages of phagocytosed yeast cells killed after specific times. (b) Studies investigating transmigration across the blood-brain barrier demonstrated that significantly (P < 0.001; t test) more NP1-SM than NP1-MC colonies could be recovered from brains of i.v. injected mice.