TABLE 2.
Effect of IL-6 in combination with sIL-6Rα on cell surface expression of ICAM-1 and U937 adherence
| IL-6 concn (ng ml−1) | sIL-6Rα concn (ng ml−1) | % ICAM-1 expressiona | % U937 adherenceb |
|---|---|---|---|
| 0 | 0 | 8.9 ± 2.3 (1) | 7.6 ± 1.9 (1) |
| 1 | 0 | 11.4 ± 2.6 (1.28) | 10.1 ± 2.1 (1.33) |
| 10 | 0 | 14.2 ± 4.8 (1.6) | 12.1 ± 1.5 (1.59) |
| 20 | 0 | 14.5 ± 1.6 (1.63) | 13.9 ± 2.7 (1.83) |
| 0 | 20 | 17.7 ± 7.5 (1.99) | 15.5 ± 6.9 (1.99) |
| 1 | 20 | 20.4 ± 6.7 (2.29) | 18.4 ± 7.6 (2.42) |
| 10 | 20 | 22.3 ± 2.0 (2.51) | 20.0 ± 4.7 (2.63) |
| 20 | 20 | 25.8 ± 5.1 (2.9) | 21.1 ± 6.1 (2.9) |
a
The percentage of adhesion molecule expressed on the cells was determined by flow cytometry. The data represent the mean ± standard deviation of three independent experiments.
b
Baseline or constitutive expression was normalized, and the increase (fold) in the expression in response to bacteria was calculated.