FIG. 4.

Conventional Western blotting used for validation of PowerBlot data. Freshly elutriated human monocytes were cultured and treated with P. gingivalis or with its purified components, and their total protein fractions were extracted. Samples containing ∼50 μg protein were subjected to Western blotting as described in Materials and Methods. Each blot was rehybridized with antiactin antibodies to compare loadings in different lanes. The results of chemiluminescence analysis of the blots are presented. The numbers above the lanes indicate average ratios relative to controls, normalized (with β-actin) using the Bio-Rad image analysis program. Asterisks indicate changes that were statistically significant, as determined by the Student t test (P < 0.05; n = 3). P.g., P. gingivalis.