TABLE 1.
Comparison of cytokine levels obtained by cytokine antibody array analysis and by ELISA
| Cell culture treatment | Level (mean ± SD) ofa:
|
|||||
|---|---|---|---|---|---|---|
| TNF-α
|
IL-6
|
IL-10
|
||||
| Cytokine array (odu/mm2) | ELISA (pg/ml) | Cytokine array (odu/mm2) | ELISA (pg/ml) | Cytokine array (odu/mm2) | ELISA (pg/ml) | |
| None | — | 34 ± 12 | — | 54 ± 21 | — | 28 ± 14 |
| LPS | 79.6 ± 8.2 | 1,493 ± 42 | 253.3 ± 32.5 | 4,244 ± 166 | 102.3 ± 8.6 | 1,992 ± 104 |
| FimA | 59.6 ± 8.6 | 996 ± 84 | 236.4 ± 48.1 | 3,445 ± 82 | 84.8 ± 14.2 | 1,532 ± 98 |
| Live P.g. | 102.4 ± 42.8 | 1,943 ± 238 | 272.3 ± 26.4 | 4,435 ± 296 | 126.9 ± 17.1 | 2,231 ± 312 |
a
The cytokine spot intensities in array membranes were quantified by optical density unit (odu) with Bio-Rad VersaDoc Imaging System 3000 and analyzed with Quantity One software. —, no spot detected in the array membrane.