FIG. 3.

In vitro dimerization of full-length RNA (RNA-4) is inhibited by antisense DNA oligonucleotides complementary to the DIS sequences. (A) Antisense DNA oligomers complementary to various regions of the Mo-MuLV 5′ UTR were designed; their properties are detailed in Table 1. PBS, primer binding site. (B) α-³²P-labeled RNA-4 (1.5 nM) was incubated with 60 μM concentrations of various unlabeled antisense oligomers at 60°C for 1 h and then analyzed on a 5% nondenaturing polyacrylamide gel. Dimerization of RNA-4 was inhibited only by oligomers that overlapped portions of the predicted palindromes in DIS-1 or DIS-2. D, dimeric RNA; M, monomeric RNA. αDIS-1, αDIS-2, and αPBS, antisense oligonucleotides complementary to DIS-1, DIS-2, and PBS sequences, respectively.