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. 2002 Apr;76(7):3158–3167. doi: 10.1128/JVI.76.7.3158-3167.2002

FIG. 3.

FIG. 3.

Expression of IE19 protein. The expression vector for IE19 (pME-IE19) or IE72 (pME-IE72) or the control vector (pME) was transfected into HeLa cells, and the expression of each IE protein was examined by Western blotting using anti-IE protein MAb 810 (lanes 1 to 3). The plasmid vector expressing IE19-FLAG fusion protein (pME-IE19FLAG) or the control plasmid was transfected into HEL cells. Expression of FLAG protein was examined by Western blotting with anti-FLAG antibody (lanes 4 and 5). HEL cells infected with HCMV (Towne) at an MOI of 5 were harvested at 24 hpi, and the expression of IE proteins was examined with MAb 810 (lanes 6 and 7). The positions of IE86, IE72, and IE19 proteins are indicated.