FIG. 6.

Activation of the HsOrc1 gene by HCMV infection. (A) Expression of HsOrc1 mRNA in HCMV-infected cells was examined by Northern blot hybridization. Poly(A)⁺ mRNA (18 μg) of HCMV-infected or mock-infected HEL cells (48 hpi) was applied to each lane. An HsOrc1 cDNA probe was used for the hybridization. (B) Expression of HsOrc1 mRNA was examined by RT-PCR. The same preparation of poly(A)⁺ mRNA (0.2 μg) used in Northern analysis (A) was examined by RT-PCR with HsOrc1-specific primers. As a control, expression of β-actin mRNA was also examined. The PCR cycle was repeated 20 times. (C) Kinetics of the HsOrc1 gene activation was examined by RT-PCR. Total RNA of HCMV-infected HEL cells (0.2 μg) was used for RT-PCR. The PCR cycle was repeated 40 times. The RNA samples are described in the legend to Fig. 5B. The nucleotide sequences of these RT-PCR primers and probes are shown in Table 1.