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Two-dimensional gel electrophoresis of Hirt DNA from BPV-1-infected S. cerevisiae cultures. A total of 10 μg of Hirt DNA was electrophoresed as described in the text, blotted onto nylon, and hybridized with a BPV-1 DNA probe. The left panel shows the hybridization results of the first- and second-dimensional blots, and the right panel shows the diagrammed pattern of the replication intermediates.
