FIG. 7.
Comparison of the abilities of the various HIV-1 RTs (mutant and wild-type [WT]) to excise AZTMP from the end of the primer and extend it in the presence of AZTTP and various concentrations of sodium pyrophosphate (NaPPi). These reactions are quite similar to the reactions whose results are depicted in Fig. 5; however, in this case, the pyrophosphate donor is pyrophosphate. (A) Reactions run in the presence of 10 μM concentrations of each of the dNTPs. (B) Reactions run in the presence of 100 μM concentrations of each of the dNTPs.