FIG. 8.

Relative efficiency of AZTMP incorporation or excision at various positions. The abilities of the various HIV-1 RTs to be blocked by AZTMP incorporation at various positions on an M13mp18 template were compared at various concentrations of ATP (see Materials and Methods). The primer was labeled with ³²P, and the reaction products were fractionated by electrophoresis on a 6% polyacrylamide gel. On the left is a scale showing the sizes of the DNA products. Arrows on the right indicate positions at which the excision efficiency differs for the RT mutants.