FIG. 2.

Wild-type and mutant E1 proteins do not associate with mitotic chromosomes. The transformed cell lines described in Fig. 1 were used here. Wild-type and mutant E1 proteins were detected by confocal microscopy using monoclonal antibody BPV104, followed by Cy3-conjugated Ig secondary incubation (green). Red, DAPI staining of chromosomal DNA. Bar, 10 μm. We could not detect E1 by these cytological methods in wild-type BPV-1-transformed cells. Thus, the E2-A3 variant, which transforms stably and shows no segregation defects, served as the wild-type control. Control, nontransformed C127 anaphase cell.