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E2-A4 protein by itself colocalizes with mitotic chromosomes. COS-7 cells were infected with the recombinant SV40 virus PAVAE2-A4, and after 24 h the E2-A4 protein was detected as described for Fig. 3. An infected anaphase cell is shown by fluorescence microscopy, using a Zeiss axioplan fluorescence microscope. Lower panel, E2-A4 immunofluorescence (yellow); upper panel, DAPI staining (blue). Bar, 10 μm.
