FIG. 1.

Schematic for nucleoprotein complex assembly and the half-site and full-site integration reactions. A radioactive 5′-end-labeled linear DNA donor (0.48 kbp) containing 3′ OH recessed U5 and U3 LTR termini is assembled with IN on ice prior to the addition of supercoiled target DNA (2.8 kbp) (40). Half-site integration involves the insertion of one LTR end per target, and full-site integration involves the concerted insertion of two LTR ends per target. Restriction enzyme analysis resolves donor-target products.