FIG. 4.

HSV-induced cell protrusions. (A) Typical example of a Vero cell infected with HSV-1 166v where highly extended protrusions containing GFP-VP22 vesicles can be seen extending to and making intimate contact with adjacent cells. Arrows indicate the membrane extensions coming from one cell (cell no. 1). (B) A high-magnification image of a section of an infected cell containing protrusions and stained for nonmuscle myosin II. Filaments containing NMIIA can be detected running through the protrusions (arrows, left panel). GFP-VP22-containing particles (arrows, center panel) align along nonmuscle myosin II filaments within the protrusions.