FIG. 6.

BDM has no significant effect on ongoing virus protein synthesis. (A) Vero cells were mock infected (M) or infected with HSV-1 (MOI of 10), and after 1 h (t = 0), medium was replaced with medium containing no BDM (−) or 10 mM BDM (+). Labeling medium containing ³⁵S-methionine (including BDM where appropriate) was added to cells at the indicated times, and incubation continued for 1 h. Cells were then harvested and analyzed by SDS-PAGE and autoradiography. Asterisks indicate the position of major infected cell-specific proteins. (B) As for panel A, except that the cells were extracted in RIPA buffer and VP22 was immunoprecipitated using a specific polyclonal antibody. The two bands represent differentially phosphorylated forms of VP22.