FIG. 2.

A region of the PAN promoter is specifically responsive to ORF 50 protein. (A) Sequence of the PAN promoter from −107 to −1; •, recently mapped start of PAN RNA (28). (B) Sequence of the adenovirus E4 minimal promoter. Potential binding sites for transcription factors identified by computer search are boxed, as is the KSHV/ORF 50 response element identified by mutagenesis experiments (Fig. 1). [ ], position where fragments of PANp or K12P were inserted. (C and D) Response of CAT reporters containing the KSHV PAN promoter and the promoters of the EBV BaRF1 (ap) and BRLF1 (Rp) genes to KSHV ORF 50 protein (KSHV/ORF50) or EBV BRLF1 protein (EBV/Rta) in BJAB (C) and 293T (D) cells.