Skip to main content
. 2002 Apr;76(7):3168–3178. doi: 10.1128/JVI.76.7.3168-3178.2002

FIG. 3.

FIG. 3.

Sequence in the promoter of the K12 gene that is responsive to ORF 50 protein. (A) Comparison of sequences in the ORF 50 protein response element in the PAN promoter with a region of the K12 promoter. Identical sequences are shown by vertical lines. The sequence in the K12 mutant are boxed. Arrowheads demarcate the minimal region of the two promoters that is responsive to ORF 50 protein. (B) Response of PAN (−91 to −58), K12 (−105 to −72), and K12 mutant (K12-MT) in E4CAT to stimulation by ORF 50 protein. (C) Effect of deletions of the K12 promoter cloned in E4CAT on its response to ORF 50 protein and ORF 50(1-490)+VP. The deletions are designated by the number of nucleotides removed from the 3′ end (e.g., 3′-D4 removed 4 nt) or 5′ end. K12-S, short K12p; S.D., standard deviation. For panels B and C, the assays were performed in BJAB cells. The capacity of the duplex oligonucleotide to bind ORF 50(1-490)+VP was determined in competition EMSAs. (−, no competition; ++++, strongest competition).