FIG. 5.
Activation of viral transcripts by ORF 50(1-490)+VP. HH-B2 cells were transfected with plasmids expressing empty vector (pRTS), full-length ORF 50 protein (ORF 50), ORF 50(1-490)+VP, or ORF 50(1-490). Uninduced and n-butyrate-treated cells were negative and positive controls for lytic gene expression. Northern blots were probed with the PAN RNA gene (A) or an oligonucleotide from the K12 gene (B).