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. 2002 Apr;76(7):3168–3178. doi: 10.1128/JVI.76.7.3168-3178.2002

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Copyright © 2002, American Society for Microbiology

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FIG. 6. — The N-terminal 490 aa of ORF 50 protein fused to VP16 binds to the promoters of the KSHV PAN and K12 genes. Extracts of HKB5/B5 cells transfected with deletion mutants of ORF 50 protein fused to VP16 were used in EMSAs. Antibody to VP16 (αVP) was used to supershift the complexes formed. The probes were the PAN promoter (−91 to −58) (A) and the K12 promoter (−105 to −72) (B). The ORF 50-specific complexes and the supershifted (SS) complex are indicated. (C) Comparison of DNA binding by ORF 50(1-490) and ORF 50(1-490)+VP to PANp. The binding reactions were supershifted with antibody to ORF 50(230-250) or with antibody to VP16.

FIG. 6. — The N-terminal 490 aa of ORF 50 protein fused to VP16 binds to the promoters of the KSHV PAN and K12 genes. Extracts of HKB5/B5 cells transfected with deletion mutants of ORF 50 protein fused to VP16 were used in EMSAs. Antibody to VP16 (αVP) was used to supershift the complexes formed. The probes were the PAN promoter (−91 to −58) (A) and the K12 promoter (−105 to −72) (B). The ORF 50-specific complexes and the supershifted (SS) complex are indicated. (C) Comparison of DNA binding by ORF 50(1-490) and ORF 50(1-490)+VP to PANp. The binding reactions were supershifted with antibody to ORF 50(230-250) or with antibody to VP16.