Table 2. In vitro furin cleavage of immature pseudovirus bypasses the requirement for cellular furin.
| Pseudovirus added, μl
|
||||
|---|---|---|---|---|
| FI | 1.25 | 0.63 | 0.32 | 0.16 |
| untx. iPV | ||||
| − | 30.7 | 17.9 | 14.1 | 7.4 |
| + | 0.4 | 0.2 | 0.1 | 0.1 |
| furin-tx. iPV | ||||
| − | 29.9 | 20.2 | 10.7 | 8.1 |
| + | 11.6 | 4.6 | 2.2 | 0.8 |
| untx. mPV | ||||
| − | 28.7 | 17.2 | 14.9 | 6.3 |
| + | 1.2 | 0.2 | 0.1 | 0.2 |
| furin-tx. mPV | ||||
| − | 29.2 | 17.4 | 14.9 | 6.0 |
| + | 1.6 | 0.6 | 0.4 | 0.3 |
Immature (iPV) or mature (mPV) BPV1 pseudoviruses were either treated with furin (furin-tx.) or left untreated (untx.). The infectivity of these preparations was evaluated on untreated HeLa cells (−) or HeLa cells treated with furin inhibitor (+). The percent of GFP-positive cells is shown. FI, furin inhibition.