Fig. 2.

Effect of leptin treatment on CUS-induced reduction in sucrose preference. (A) Sucrose preference expressed as a ratio of the volume of sucrose intake to the volume of water intake. CUS rats display a substantially decreased sucrose preference. This effect of CUS was reversed by leptin treatment (1 mg/kg, i.p.). ANOVA revealed a significant effect on sucrose preference for time after injection [F(1,26) = 37.38, P < 0.0001] and drug treatment [F(2,13) = 7.498, P < 0.01]. ^**, P < 0.01 compared with controls. (B) Total amount of fluid intake. The total fluid intake was not affected by leptin treatment. Rats subjected to CUS significantly drink less than handled controls [F(1,28) = 6.525, P < 0.05]. ^*, P < 0.05. The empty underbar indicates measurements in the light cycle; the filled underbar shows measurements in the dark cycle.