| 1 |
30-min restraint |
a.m. |
| 2 |
1-h shaking/crowding, 240 Hz with 6 rats in a box (1 ft × 1 ft × 1 ft) |
p.m. |
| 3 |
24-h separation |
|
| 4 |
15-min warm swim (31°C) |
a.m. |
| 5 |
24-h high density of housing (6 rats per cage) |
|
| 6 |
10-min cold swim (18°C) |
p.m. |
| 7 |
1.5-h shaking/crowding, 240 Hz with 6 rats in a box (1 ft × 1 ft × 1 ft) |
a.m. |
| 8 |
10-min inescapable shock (1.5 mA, 15 s on, 150 s off) |
p.m. |
| 9 |
24-h separation |
|
| 10 |
15-min warm swim (31°C) |
a.m. |
| 11 |
15-min inescapable shock (1.5 mA, 15 s on, 150 s off) |
p.m. |
| 12 |
10-min tail pinch (a clothespin placed at 1 cm from the base of tail) |
a.m. |
| 13 |
10-min cold swim (18°C) |
p.m. |
| 14 |
15-min inescapable shock (1.5 mA, 15 s on, 150 s off) |
a.m. |
