Fig. 2.

PPG-inferred extension of the last exon in GNA11. Shown is a snapshot from the University of California, Santa Cruz, genome browser [http://genome.ucsc.edu/cgi-bin/hgGateway (hg17)]. Boxes depict exons and thin arrowed lines are introns. The “Blat search” track shows the alignment of the PPG to its gene of origin. Index numbers of exons are marked above the track. The last exon (exon 7) in the Refseq of guanine nucleotide binding protein alpha 11 (GNA11) contains 466 bp. The PPG chr7_P29992.1 shows a 2,539-bp extension of the last Refseq exon when aligned to the GNA11 locus. No polyadenylation signal appears near the end of the Refseq's last exon, but a genomic A-rich sequence (underlined) appears immediately downstream the Refseq terminus, implying that the Refseq sequence was primed from this internal genomic poly(A). A canonical polyadenylation signal is found 12 bp upstream the PPG-inferred end (italicized and underlined), indicating that this is probably the real terminus of the GNA11 mRNA.