FIG. 2.

ORF2 protein is present in the membrane fraction of VZV-infected cells but not in virions. Membrane and cytosolic fractions from VZV-infected cells were prepared, and immunoblotting shows a band of 31-kDa corresponding to the ORF2 protein in the membrane fraction of VZV-infected cells (A). Immunoblotting with antibody to VZV gE shows gE also in the membrane fraction of infected cells (B). Cell lysates from VZV-infected cells, uninfected cells, or purified VZV virions were boiled in sample buffer, and immunoblotting was performed with antibodies (21, 25) to VZV ORF2 protein (C), ORF10 protein (D), and ORF61 protein (E). The numbers to the right of the blots indicate molecular masses (in kilodaltons).