TABLE 3.

Percentage of H6LT-expressing cells and copy number of each H6LT^a

Transcript	% Positive	Copy number/cell (copy number/H6LT-positive cell)
Type I IE1-related	1.4 ± 0.4	0.49 ± 0.36 (35 ± 26)
Type I IE2-related	4.7 ± 3.8	2.8 ± 0.75 (60 ± 16)
Type II IE1-related	7.4 ± 3.1	5.3 ± 3.2 (72 ± 43)
Type II IE2-related	2.3 ± 1.1	0.87 ± 0.62 (38 ± 27)
Productive-phase IE1	4.3 ± 0.75b	35.8 ± 14.4 (833 ± 335)c

^aPerformed on four independent macrophage cultures. RT-PCR was performed at least four times for each cell dilution, and the percentages of H6LT-positive cells in the HHV-6 DNA-positive cells were estimated by the Reed-Muench method shown in Fig. 6A. The copy number of the H6LT was also estimated by the cDNA dilution study (Fig. 6B). The copy number of each H6LT per H6LT-positive cell is shown. The average of four independent cultures ± standard deviation is given for each latent transcript.

^bPercentage of IE1-positive cells in total cells.

^cCopy number of IE1 in a single IE1-positive cell.