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(A) FAK was immunoprecipitated (Ip) from clone 43 (+/−) or 17 (−/−) cells and Western-blotted (Wb) with antibodies against phosphotyrosine (pY20 and 4G10) and FAK as indicated. (B) Whole-cell lysates from clone 43 or 17 cells were probed with the indicated antibodies. FAK is minimally phosphorylated at Tyr397 and Tyr861 in clone 17 (−/−) cells.
