Figure 1. mBB reactivity of IP₃R in cerebellum microsomes.

IP₃Rs immunoprecipitated from cerebellum microsomes were allowed to react for 10 min in the presence or absence of 5 mM 2ME and 1 mM NEM. The samples were then denatured and incubated with 2 mM mBB for 20 min as described in detail in the Experimental section. After electrophoresis the gels were fixed and visualized for fluorescently labelled IP₃R and for IP₃R protein by Coomassie Blue staining.