Table 1. Primers used for cysteine mutagenesis.

The mutated codon is underlined.

Mutant	Primers
	Sense (5′→3′)	Antisense (3′→5′)
C15S	5′ catatcggagacatttcctctctgtatgcagag	3′ gtatagcctctgtaaaggagagacatacgtctc
C37S	5′ ttggttgatgaccgttccgttgtacagccagaa	3′ aaccaactactggcaaggcaacatgtcggtctt
C57/61S	5′ ttcagagactccctctttaagctatcccctatgaat	3′ aagtctctgagggagaaattcgataggggatagtta
C206/214S	5′ cgggctccaatgaggtcaactccgtcaactccaacaca	3′ gcccgaggttactccagttgaggcagttgaggttgtgt
C253S	5′ gagaagtttctcacgtccgatgagcacaggaag	3′ gtgttcaaagagtgcaggctactcgtgtccttc
C292S	5′ gtccagcatgacccatcccggggtggagctggg	3′ caggtcgtactgggtagggccccacctcgaccc
C326S	5′ gactttgaggaagaatccctggagtttcagccg	3′ ctgaaactccttcttagggacctcaaagtcggc