Figure 1. Effects on the expression and secretion of representative LEE proteins when the initiation codon of l0036 was altered.

Bacteria transformed with (+) or without (−) pQL36 were cultivated in M9 medium for 6 h. Bacterial lysates and proteins from spent medium were then prepared and analysed by Western blotting using antibodies specific to the antigen indicated. Abbreviations: WT, the wild-type strain 43888; AC36, a mutant strain 43888 with initiation codon of l0036 altered from ATG to CTG.