Figure 2. LEE3 genes downstream of l0036 required for AC36 to complement the defect in the type III secretion.

(A) Schematic of gene organization (not to scale) flanking l0036 and illustration of the DNA inserts in the complementation plasmids. (B) Proteins from bacterial lysates and spent medium were analysed as described above in the legend to Figure 1. O157, the antigen detected by anti-O157 antibodies from Difco.