Figure 5. Ler is involved in the effects of L0036.

(A) Counteracting effect of L0036 on the activation of Ler on the LEE expression. Effects of expressing L0036 or L0044 in addition to Ler were examined by analysis of the LEE proteins in a Ler-deleted mutant, strain 43888 ΔLer. The mutant strain receiving various plasmids were analysed for the LEE proteins expressed in the bacterial lysates as described in the legend to Figure 1. (B) Interaction of Ler and L0036 as shown by a bacterial two-hybrid system. ORFs were cloned separately into expression plasmids to serve as target protein and bait. The interactions of the target and bait proteins in a reporter strain were revealed by an increase in colony number on a carbenicillin plate or by an increase in the bacterial β-galactosidase activity. Note: the known interaction between the dimerization domain of yeast transcriptional activator Gal4 (LGF2) and a domain derived from a mutant form of GAL11 (Stratagene) served as a positive control.