Table 1. Fluorescence maxima (nm), relative intensities (peak area, arbitrary units) and emission lifetimes (ns) for wild-type (WT) and W128F mutant CYP2D6 and MAMC.
For the lifetimes, the relative contributions to the total signal are indicated in parentheses (%). Iem is the emission intensity. Results are means±S.D.
| λex (nm) | λem (nm) | Iem (area) | τ1 (ns) | τ2 (ns) | τ3 (ns) | |
|---|---|---|---|---|---|---|
| WT | 278 | 336 | 1.38±0.003 | 0.13±0.05 (80%) | 1.06±0.03 (17%) | 3.81±0.08 (3%) |
| WT+MAMC | 278 | 335 | 1.28±0.006 | 0.12±0.06 (83%) | 1.30±0.27 (14%) | 4.00±0.17 (3%) |
| W128F | 280 | 338 | 1.07±0.002 | 0.07±0.02 (84%) | 1.09±0.06 (13%) | 4.17±0.06 (3%) |
| W128F+MAMC | 280 | 333 | 0.99±0.004 | 0.14±0.04 (81%) | 1.40±0.24 (16%) | 3.96±0.30 (3%) |
| MAMC | 328 | 406 | 0.56±0.018 | 0.75±0.12 (52%) | 2.53±0.06 (48%) | |
| MAMC+WT | 328 | 400 | 0.36±0.006 | 0.68±0.07 (56%) | 2.62±0.11 (44%) | |
| MAMC+W128F | 327 | 400 | 0.36±0.006 | 0.71±0.16 (50%) | 2.77±0.17 (50%) |